RNA 3-dimensional structural motifs as a critical constraint of viroid RNA evolution
نویسندگان
چکیده
1 Department of Biological Sciences, Mississippi State University, Starkville, Mississippi, United States of America, 2 Department of Mathematics and Statistics, Bowling Green State University, Bowling Green, Ohio, United States of America, 3 Department of Chemistry and Center for Biomolecular Sciences, Bowling Green State University, Bowling Green, Ohio, United States of America, 4 Department of Molecular Genetics, The Ohio State University, Columbus, Ohio, United States of America, 5 The Center for RNA Biology, The Ohio State University, Columbus, Ohio, United States of America
منابع مشابه
A three-dimensional RNA motif in Potato spindle tuber viroid mediates trafficking from palisade mesophyll to spongy mesophyll in Nicotiana benthamiana.
Cell-to-cell trafficking of RNA is an emerging biological principle that integrates systemic gene regulation, viral infection, antiviral response, and cell-to-cell communication. A key mechanistic question is how an RNA is specifically selected for trafficking from one type of cell into another type. Here, we report the identification of an RNA motif in Potato spindle tuber viroid (PSTVd) requi...
متن کاملTertiary structural and functional analyses of a viroid RNA motif by isostericity matrix and mutagenesis reveal its essential role in replication.
RNA-templated RNA replication is essential for viral or viroid infection, as well as for regulation of cellular gene expression. Specific RNA motifs likely regulate various aspects of this replication. Viroids of the Pospiviroidae family, as represented by the Potato spindle tuber viroid (PSTVd), replicate in the nucleus by utilizing DNA-dependent RNA polymerase II. We investigated the role of ...
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As a consequence of two unique physical properties, small size and circularity, viroid RNAs do not code for proteins and thus depend on RNA sequence/structural motifs for interacting with host proteins that mediate their invasion, replication, spread, and circumvention of defensive barriers. Viroid genomes fold up on themselves adopting collapsed secondary structures wherein stretches of nucleo...
متن کاملA genomic map of viroid RNA motifs critical for replication and systemic trafficking.
RNA replication and systemic trafficking play significant roles in developmental regulation and host-pathogen interactions. Viroids are the simplest noncoding eukaryotic RNA pathogens and genetic units that are capable of autonomous replication and systemic trafficking and offer excellent models to investigate the role of RNA structures in these processes. Like other RNAs, the predicted seconda...
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Viroids are noncoding RNAs that infect plants. In order to establish systemic infection, these RNAs must traffic from an initially infected host cell into neighboring cells and ultimately throughout a whole plant. Recent studies have identified structural motifs in a viroid that are required for trafficking, enabling further studies on the mechanisms of their function. Some cellular proteins in...
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